Multi-micron DNA structures assembled from crisscross DNA slats is a recently developed technique that has pushed DNA origami to the micron scale using hierarchical joining and nucleation1. Crisscross polymerization relies on 6HB or 12HB DNA origami with weak binding handles as the monomer unit. By adding a seed that nucleates growth, crisscross polymerization is able to realize both finite and infinite assembly of large micron scale structures. This technique has been used to create megastructures with high yield of 2 µm × 2 µm with over 10,000 addressable nodes. This technique has advanced the size of addressable DNA nanostructures possible. However, these structures are mostly limited to 2D.
References
- Wintersinger, C. M. et al. Multi-micron crisscross structures grown from DNA-origami slats. Nat. Nanotechnol. 18, 281–289 (2023).